LSBio
RPUSD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RPUSD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105274-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPUSD3 (NM_173659), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RPUSD3
Origin Species
Human
NCBI GenBank
AA703931
SwissProt
Q6P087

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MDGRRVLGRF WSGWRRGLGV RPVPEDAGFG TEARHQRQPR GSCQRSGPLG DQPFAGLLPK
61NLSREELVDA LRAAVVDRKG PLVTLNKPQG LPVTGKPGEL TLFSVLPELS QSLGLREQEL
121QVVRASGKES SGLVLLSSCP QTASRLQKYF THARRAQRPT ATYCAVTDGI PAASEGKIQA
181ALKLEHIDGV NLTVPVKAPS RKDILEGVKK TLSHFRVVAT GSGCALVQLQ PLTVFSSQLQ
241VHMVLQLCPV LGDHMYSARV GTVLGQRFLL PAENNKPQRQ VLDEALLRRL HLTPSQAAQL
301PLHLHLHRLL LPGTRARDTP VELLAPLPPY FSRTLQCLGL RLQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

343 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.