LSBio
RPUSD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RPUSD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106408-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPUSD2 (NM_152260), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C15orf19 | RPUSD2 | C18B11
Origin Species
Human
NCBI GenBank
BC007697
SwissProt
Q8IZ73

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWLDRRGWLR VLGHWRYDLR RPSFTRTWSG DKGPMAETVS TQVGTEGGLR ASHQQNGDAG
61GDAKVELSPG PPKPAGREVE PAPVGGEHPS AAAPGPGKHK KRRGATRERV VPPPKKRRTG
121VSFGDEHFAE TSYYFEGGLR KVRPYYFDFR TYCKGRWVGH SLLHVFSTEF RAQPLAYYEA
181AVRAGRLQLN EKPVQDLNIV LKDNDFLRNT VHRHEPPVTA EPIRLLAENE DVVVVDKPSS
241IPVHPCGRFR HNTVIFILGK EHQLKELHPL HRLDRLTSGV LMFAKTAAVS ERIHEQVRDR
301QLEKEYVCRV EGEFPTEEVT CKEPILVVSY KVGVCRVDPR GKPCETVFQR LSYNGQSSVV
361RCRPLTGRTH QIRVHLQFLG HPILNDPIYN SVAWGPSRGR GGYIPKTNEE LLRDLVAEHQ
421AKQSLDVLDL CEGDLSPGLT DSTAPSSELG KDDLEELAAA AQKMEEVAEA APQELDTIAL
481ASEKAVETDV MNQETDPLCA ECRLVRQDPL PQDLVMFLHA LRYKGPGFEY FSPMPAWAQD
541DWQKD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.