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RPS6KL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RPS6KL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102003-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPS6KL1 (NM_031464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RPS6KL1
Origin Species
Human
NCBI GenBank
NM_031464
SwissProt
Q9Y6S9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLVACECLP SPGLEPEPCS RARSQAHVYL EQIRNRVALG VPDMTKRDYL VDAATQIRLA
61LERDVSEDYE AAFNHYQNGV DVLLRGIHVD PNKERREAVK LKITKYLRRA EEIFNCHLQR
121PLSSGASPSA VQLVQDPATG GTFVVKSLPR CHMVSRERLT IIPHGVPYMT KLLRYFVSED
181SIFLHLEHVQ GGTLWSHLLS QAHSRHSGLS SGSTQERMKA QLNPHLNLLT PARLPSGHAP
241GQDRIALEPP RTSPNLLLAG EAPSTRPQRE AEGEPTARTS TSGSSDLPKA PGGHLHLQAR
301RAGQNSDAGP PRGLTWVPEG AGPVLGGCGR GMDQSCLSAD GAGRGCGRAT WSVREEQVKQ
361WAAEMLVALE ALHEQGVLCR DLHPGNLLLD QAGHIRLTYF GQWSEVEPQC CGEAVDNLYS
421APEVGGISEL TEACDWWSFG SLLYELLTGM ALSQSHPSGI QAHTQLQLPE WLSRPAASLL
481TELLQFEPTR RLGMGEGGVS KLKSHPFFST IQWSKLVGTS LETHLRTLIH WLSGPKPDCP
541G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

541 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.