Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPP40 / Ribonuclease P Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115176-100
Catalog Number: LS-G115176
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPP40 (NM_006638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA428J1.3 | Ribonuclease P | 40kD subunit | RNASEP1 | Ribonuclease P (40 kD) | Ribonuclease P 40kDa subunit | Ribonuclease P1 | RNase P subunit 1 | RNaseP protein p40 | RPP40
- Origin Species
- Human
- NCBI GenBank
- NM_006638
- SwissProt
- O75818
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG ILSEELKNLV61MNTGPYYFVK NLPLHELITP EFISTFIKKG SCYALTYNTH IDEDNTVALL PNGKLILSLD121KDTYEETGLQ GHPSQFSGRK IMKFIVSIDL MELSLNLDSK KYERISWSFK EKKPLKFDFL181LAWHKTGSEE STMMSYFSKY QIQEHQPKVA LSTLRDLQCP VLQSSELEGT PEVSCRALEL241FDWLGAVFSN VDLNNEPNNF ISTYCCPEPS TVVAKAYLCT ITGFILPEKI CLLLEHLCHY301FDEPKLAPWV TLSVQGFADS PVSWEKNEHG FRKGGEHLYN FVIFNNQDYW LQMAVGANDH361CPPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
363 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.