Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPH3A / Rabphilin 3A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113588-100
Catalog Number: LS-G113588
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rabphilin 3A(RPH3A) (NM_014954), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0985 | Rabphilin 3A | RPH3A | Exophilin-1 | Rabphilin | Rabphilin 3A homolog (mouse) | Rabphilin-3A
- Origin Species
- Human
- SwissProt
- Q9Y2J0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTDTVFSNSS NRWMYPSDRP LQSKLQAGWS VHPGGQPDRQ RKQEELTDEE KEIINRVIAR61AEKMEEMEQE RIGRLVDRLE NMRKNVAGDG VNRCILCGEQ LGMLGSACVV CEDCKKNVCT121KCGVETNNRL HSVWLCKICI EQREVWKRSG AWFFKGFPKQ VLPQPMPIKK TKPQQPVSEP181AAPEQPAPEP KHPARAPARG DSEDRRGPGQ KTGPDPASAP GRGNYGPPVR RASEARMSSS241SRDSESWDHS GGAGDSSRSP AGLRRANSVQ ASRPAPGSVQ SPAPPQPGQP GTPGGSRPGP301GPAGRFPDQK PEVAPSDPGT TAPPREERTG GVGGYPAVGA REDRMSHPSG PYSQASAAAP361QPAAARQPPP PEEEEEEANS YDSDEATTLG ALEFSLLYDQ DNSSLQCTII KAKGLKPMDS421NGLADPYVKL HLLPGASKSN KLRTKTLRNT RNPIWNETLV YHGITDEDMQ RKTLRISVCD481EDKFGHNEFI GETRFSLKKL KPNQRKNFNI CLERVIPMKR AGTTGSARGM ALYEEEQVER541VGDIEERGKI LVSLMYSTQQ GGLIVGIIRC VHLAAMDANG YSDPFVKLWL KPDMGKKAKH601KTQIKKKTLN PEFNEEFFYD IKHSDLAKKS LDISVWDYDI GKSNDYIGGC QLGISAKGER661LKHWYECLKN KDKKIERWHQ LQNENHVSSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
690 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.