Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPF2 / BXDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108979-100
Catalog Number: LS-G108979
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BXDC1(RPF2) (NM_032194), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA397G5.4 | BXDC1 | Homolog of Rpf2 | RPF2 | Brix domain containing 1
- Origin Species
- Human
- NCBI GenBank
- NM_032194
- SwissProt
- Q9H7B2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTLDRVVKP KTKRAKRFLE KREPKLNENI KNAMLIKGGN ANATVTKVLK DVYALKKPYG61VLYKKKNITR PFEDQTSLEF FSKKSDCSLF MFGSHNKKRP NNLVIGRMYD YHVLDMIELG121IENFVSLKDI KNSKCPEGTK PMLIFAGDDF DVTEDYRRLK SLLIDFFRGP TVSNIRLAGL181EYVLHFTALN GKIYFRSYKL LLKKSGCRTP RIELEEMGPS LDLVLRRTHL ASDDLYKLSM241KMPKALKPKK KKNISHDTFG TTYGRIHMQK QDLSKLQTRK MKGLKKRPAE RITEDHEKKS301KRIKKNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
306 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.