Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPAP3 / FLJ21908 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128193-100
Catalog Number: LS-G128193
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RPAP3 (NM_001146076), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLJ21908 | RPAP3 | Spag
- Origin Species
- Human
- NCBI GenBank
- NM_024604
- SwissProt
- Q9H6T3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDADPYNPVL PTNRASAYFR LKKFAVAESD CNLAVALNRS YTKAYSRRGA ARFALQKLEE61AKKDYERVLE LEPNNFEATN ELRKISQALA SKENSYPKEA DIVIKSTEGE RKQIEAQQNK121QQAISEKDRG NGFFKEGKYE RAIECYTRGI AADGANALLP ANRAMAYLKI QKYEEAEKDC181TQAILLDGSY SKAFARRGTA RTFLGKLNEA KQDFETVLLL EPGNKQAVTE LSKIKKELIE241KGHWDDVFLD STQRQNVVKP IDNPPHPGST KPLKKVIIEE TGNLIQTIDV PDSTTAAAPE301NNPINLANVI AATGTTSKKN SSQDDLFPTS DTPRAKVLKI EEVSDTSSLQ PQASLKQDVC361QSYSEKMPIE IEQKPAQFAT TVLPPIPANS FQLESDFRQL KSSPDMLYQY LKQIEPSLYP421KLFQKNLDPD VFNQIVKILH DFYIEKEKPL LIFEILQRLS ELKRFDMAVM FMSETEKKIA481RALFNHIDKS GLKDSSVEEL KKRYGGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
506 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.