Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RORC / ROR Gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122843-100
Catalog Number: LS-G122843
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROR gamma(RORC) (NM_001001523), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Nuclear receptor ROR-gamma | Nuclear receptor RZR-gamma | RAR-related orphan receptor C | RORG | RORgamma | RORC | TOR | ROR Gamma | RZRG | NR1F3 | RZR-GAMMA
- Origin Species
- Hu
- NCBI GenBank
- NM_005060
- SwissProt
- P51449
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 55.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRTQIEVIPC KICGDKSSGI HYGVITCEGC KGFFRRSQRC NAAYSCTRQQ NCPIDRTSRN61RCQHCRLQKC LALGMSRDAV KFGRMSKKQR DSLHAEVQKQ LQQRQQQQQE PVVKTPPAGA121QGADTLTYTL GLPDGQLPLG SSPDLPEASA CPPGLLKASG SGPSYSNNLA KAGLNGASCH181LEYSPERGKA EGRESFYSTG SQLTPDRCGL RFEEHRHPGL GELGQGPDSY GSPSFRSTPE241APYASLTEIE HLVQSVCKSY RETCQLRLED LLRQRSNIFS REEVTGYQRK SMWEMWERCA301HHLTEAIQYV VEFAKRLSGF MELCQNDQIV LLKAGAMEVV LVRMCRAYNA DNRTVFFEGK361YGGMELFRAL GCSELISSIF DFSHSLSALH FSEDEIALYT ALVLINAHRP GLQEKRKVEQ421LQYNLELAFH HHLCKTHRQS ILAKLPPKGK LRSLCSQHVE RLQIFQHLHP IVVQAAFPPL481YKELFSTETE SPVGLSKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
497 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.