Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RORB / ROR Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100942-100
Catalog Number: LS-G100942
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROR beta(RORB) (NM_006914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA133M9.1 | Nuclear receptor ROR-beta | NR1F2 | ROR Beta | ROR-BETA | Rorbeta | RORB | RZR-BETA | Nuclear receptor RZR-beta | RAR-related orphan receptor B | RZRB
- Origin Species
- Human
- NCBI GenBank
- NM_006914
- SwissProt
- Q92753
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRAQIEVIPC KICGDKSSGI HYGVITCEGC KGFFRRSQQN NASYSCPRQR NCLIDRTNRN61RCQHCRLQKC LALGMSRDAV KFGRMSKKQR DSLYAEVQKH QQRLQEQRQQ QSGEAEALAR121VYSSSISNGL SNLNNETSGT YANGHVIDLP KSEGYYNVDS GQPSPDQSGL DMTGIKQIKQ181EPIYDLTSVP NLFTYSSFNN GQLAPGITMT EIDRIAQNII KSHLETCQYT MEELHQLAWQ241THTYEEIKAY QSKSREALWQ QCAIQITHAI QYVVEFAKRI TGFMELCQND QILLLKSGCL301EVVLVRMCRA FNPLNNTVLF EGKYGGMQMF KALGSDDLVN EAFDFAKNLC SLQLTEEEIA361LFSSAVLISP DRAWLIEPRK VQKLQEKIYF ALQHVIQKNH LDDETLAKLI AKIPTITAVC421NLHGEKLQVF KQSHPEIVNT LFPPLYKELF NPDCATGCKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
459 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.