RORA / ROR Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99924-100
Catalog Number: LS-G99924
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROR alpha(RORA) (NM_002943), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Nuclear receptor ROR-alpha | Nuclear receptor RZR-alpha | Roralpha | Transcription factor RZR-alpha | RAR-related orphan receptor A | ROR-alpha | ROR3 | RORA | RZRA | NR1F1 | ROR Alpha | RZR-ALPHA
- NCBI GenBank
- NM_002943
- SwissProt
- P35398
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNEGAPGDSD LETEARVPWS IMGHCLRTGQ ARMSATPTPA GEGARSSSTC SSLSRLFWSQ61LEHINWDGAT AKNFINLREF FSFLLPALRK AQIEIIPCKI CGDKSSGIHY GVITCEGCKG121FFRRSQQSNA TYSCPRQKNC LIDRTSRNRC QHCRLQKCLA VGMSRDAVKF GRMSKKQRDS181LYAEVQKHRM QQQQRDHQQQ PGEAEPLTPT YNISANGLTE LHDDLSNYID GHTPEGSKAD241SAVSSFYLDI QPSPDQSGLD INGIKPEPIC DYTPASGFFP YCSFTNGETS PTVSMAELEH301LAQNISKSHL ETCQYLREEL QQITWQTFLQ EEIENYQNKQ REVMWQLCAI KITEAIQYVV361EFAKRIDGFM ELCQNDQIVL LKAGSLEVVF IRMCRAFDSQ NNTVYFDGKY ASPDVFKSLG421CEDFISFVFE FGKSLCSMHL TEDEIALFSA FVLMSADRSW LQEKVKIEKL QQKIQLALQH481VLQKNHREDG ILTKLICKVS TLRALCGRHT EKLMAFKAIY PDIVRLHFPP LYKELFTSEF541EPAMQIDGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
548 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.