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ROBO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ROBO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ROBO2 antibodies →
$725.00each
Catalog Number: LS-G124878-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ROBO2 (NM_001128929), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1568 | ROBO2 | Roundabout homolog 2 | SAX3
Origin Species
Human
NCBI GenBank
AF040991
SwissProt
Q9HCK4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
152.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARRHERVTR RMWTWAPGLL MMTVVFWGHQ GNGQGQGSRL RQEDFPPRIV EHPSDVIVSK
61GEPTTLNCKA EGRPTPTIEW YKDGERVETD KDDPRSHRML LPSGSLFFLR IVHGRRSKPD
121EGSYVCVARN YLGEAVSRNA SLEVALLRDD FRQNPTDVVV AAGEPAILEC QPPRGHPEPT
181IYWKKDKVRI DDKEERISIR GGKLMISNTR KSDAGMYTCV GTNMVGERDS DPAELTVFER
241PTFLRRPINQ VVLEEEAVEF RCQVQGDPQP TVRWKKDDAD LPRGRYDIKD DYTLRIKKTM
301STDEGTYMCI AENRVGKMEA SATLTVRAPP QFVVRPRDQI VAQGRTVTFP CETKGNPQPA
361VFWQKEGSQN LLFPNQPQQP NSRCSVSPTG DLTITNIQRS DAGYYICQAL TVAGSILAKA
421QLEVTDVLTD RPPPIILQGP ANQTLAVDGT ALLKCKATGD PLPVISWLKE GFTFPGRDPR
481ATIQEQGTLQ IKNLRISDTG TYTCVATSSS GETSWSAVLD VTESGATISK NYDLSDLPGP
541PSKPQVTDVT KNSVTLSWQP GTPGTLPASA YIIEAFSQSV SNSWQTVANH VKTTLYTVRG
601LRPNTIYLFM VRAINPQGLS DPSPMSDPVR TQDISPPAQG VDHRQVQKEL GDVLVRLHNP
661VVLTPTTVQV TWTVDRQPQF IQGYRVMYRQ TSGLQATSSW QNLDAKVPTE RSAVLVNLKK
721GVTYEIKVRP YFNEFQGMDS ESKTVRTTEE APSAPPQSVT VLTVGSYNST SISVSWDPPP
781PDHQNGIIQE YKIWCLGNET RFHINKTVDA AIRSVIIGGL FPGIQYRVEV AASTSAGVGV
841KSEPQPIIIG RRNEVVITEN NNSITEQITD VVKQPAFIAG IGGACWVILM GFSIWLYWRR
901KKRKGLSNYA VTFQRGDGGL MSNGSRPGLL NAGDPSYPWL ADSWPATSLP VNNSNSGPNE
961IGNFGRGDVL PPVPGQGDKT ATMLSDGAIY SSIDFTTKTS YNSSSQITQA TPYATTQILH
1021SNSIHELAVD LPDPQWKSSI QQKTDLMGFG YSLPDQNKGN NGGKGGKKKK NKNSSKPQKN
1081NGSTWANVPL PPPPVQPLPG TELEHYAVEQ QENGYDSDSW CPPLPVQTYL HQGLEDELEE
1141DDDRVPTPPV RGVASSPAIS FGQQSTATLT PSPREEMQPM LQAHLDELTR AYQFDIAKQT
1201WHIQSNNQPP QPPVPPLGYV SGALISDLET DVADDDADDE EEALEIPRPL RALDQTPGSS
1261MDNLDSSVTG KAFTSSQRPR PTSPFSTDSN TSAALSQSQR PRPTKKHKGG RMDQQPALPH
1321RREGMTDEEA LVPYSKPSFP SPGGHSSSGT ASSKGSTGPR KTEVLRAGHQ RNASDLLDIG
1381YMGSNSQGQF TGEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1394 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.