Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RNLS / Renalase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111793-100
Catalog Number: LS-G111793
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Renalase(RNLS) (NM_018363), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf59 | MAO-C | Monoamine oxidase-C | Renalase | RNLS
- Origin Species
- Human
- NCBI GenBank
- NM_018363
- SwissProt
- Q5VYX0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQVLIVGAG MTGSLCAALL RRQTSGPLYL AVWDKAEDSG GRMTTACSPH NPQCTADLGA61QYITCTPHYA KKHQRFYDEL LAYGVLRPLS SPIEGMVMKE GDCNFVAPQG ISSIIKHYLK121ESGAEVYFRH RVTQINLRDD KWEVSKQTGS PEQFDLIVLT MPVPEILQLQ GDITTLISEC181QRQQLEAVSY SSRYALGLFY EAGTKIDVPW AGQYITSNPC IRFVSIDNKK RNIESSEIGP241SLVIHTTVPF GVTYLEHSIE DVQELVFQQL ENILPGLPQP IATKCQKWRH SQVPSAGVIL301GCAKSPWMMA IGFPITRTRPLEQKLISEEDLAANDILDYKDDDDKV
315 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.