Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RNFT2 / TMEM118 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108636-100
Catalog Number: LS-G108636
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM118(RNFT2) (NM_032814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TMEM118 | Transmembrane protein 118 | RNFT2
- Origin Species
- Human
- NCBI GenBank
- NM_032814
- SwissProt
- Q96EX2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLFTVNQVL RKMQRRHSSN TDNIPPERNR SQALSSEASV DEGGVFESLK AEAASPPALF61SGLSGSLPTS SFPSSLVLGS SAGGGDVFIQ MPASREEGGG RGEGGAYHHR QPHHHFHHGG121HRGGSLLQHV GGDHRGHSEE GGDEQPGTPA PALSELKAVI CWLQKGLPFI LILLAKLCFQ181HKLGIAVCIG MASTFAYANS TLREQVSLKE KRSVLVILWI LAFLAGNTLY VLYTFSSQQL241YNSLIFLKPN LEMLDFFDLL WIVGIADFVL KYITIALKCL IVALPKIILA VKSKGKFYLV301IEELSQLFRS LVPIQLWYKY IMGDDSSNSY FLGGVLIVLY SLCKSFDICG RVGGVRKALK361LLCTSQNYGV RATGQQCTEA GDICAICQAE FREPLILLCQ MLLKGHKKLE LEKIDESAGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
420 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.