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RNF207 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RNF207 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RNF207 antibodies →
$725.00each
Catalog Number: LS-G102797-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF207 (NM_207396), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf188 | RNF207 | Ring finger protein 207
Origin Species
Human
NCBI GenBank
NM_207396
SwissProt
Q6ZRF8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGAIFGPLE GPSSLDAPSI HPLVCPLCHV QYERPCLLDC FHDFCAGCLR GRATDGRLTC
61PLCQHQTVLK GPSGLPPVDR LLQFLVDSSG DGVEAVRCAN CDLECSEQDV ETTYFCNTCG
121QPLCARCRDE THRARMFARH DIVALGQRSR DVPQKCTLHA EPYLLFSTDK KLLLCIRCFR
181DMQKESRAHC VDLESAYVQG CERLEQAVLA VKALQTATRE AIALLQAMVE EVRHSAAEEE
241DAIHALFGSM QDRLAERKAL LLQAVQSQYE EKDKAFKEQL SHLATLLPTL QVHLVICSSF
301LSLANKAEFL DLGYELMERL QGIVTRPHHL RPIQSSKIAS DHRAEFARCL EPLLLLGPRR
361VAAAASGANT LAGGLGPKAL TGPHCPSPVG KMSGSPVQKP TLHRSISTKV LLAEGENTPF
421AEHCRHYEDS YRHLQAEMQS LKDQVQELHR DLTKHHSLIK AEIMGDVLHK SLQLDVQIAS
481EHASLEGMRV VFQEIWEEAY QRVANEQEIY EAQLHDLLQL RQENAYLTTI TKQITPYVRS
541IAKVKERLEP RFQAPVDEQS ESLQNTHDDS RNNAASARNN PGSVPEKREK TSEPKGNSWA
601PNGLSEEPLL KNMDHHRSKQ KNGGDVPTWR EHPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.