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RNF165 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RNF165 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106250-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF165 (NM_152470), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARKL2 | Ring finger protein 165 | RNF165
Origin Species
Human
NCBI GenBank
NM_152470
SwissProt
Q6ZSG1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MVLVHVGYLV LPVFGSVRNR GAPFQRSQHP HATSCRHFHL GPPQPQQLAP DFPLAHPVQS
61QPGLSAHMAP AHQHSGALHQ SLTPLPTLQF QDVTGPSFLP QALHQQYLLQ QQLLEAQHRR
121LVSHPRRSQE RVSVHPHRLH PSFDFGQLQT PQPRYLAEGT DWDLSVDAGL SPAQFQVRPI
181PQHYQHYLAT PRMHHFPRNS SSTQMVVHEI RNYPYPQLHF LALQGLNPSR HTSAVRESYE
241ELLQLEDRLG NVTRGAVQNT IERFTFPHKY KKRRPQDGKG KKDEGEESDT DEKCTICLSM
301LEDGEDVRRL PCMHLFHQLC VDQWLAMSKK CPICRVDIET QLGADS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

346 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.