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RNF123 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RNF123 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RNF123 antibodies →
$725.00each
Catalog Number: LS-G110499-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF123 (NM_022064), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FP1477 | KPC1 | RNF123 | Ring finger protein 123 | Ubiquitin ligase
Origin Species
Human
NCBI GenBank
NM_022064
SwissProt
Q5XPI4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
148.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASKGAGMSF SRKSYRLTSD AEKSRVTGIV QEKLLNDYLN RIFSSSEHAP PAATSRKPLN
61FQNLPEHLDQ LLQVDNEEEE SQGQVEGRLG PSTVVLDHTG GFEGLLLVDD DLLGVIGHSN
121FGTIRSTTCV YKGKWLYEVL ISSQGLMQIG WCTISCRFNQ EEGVGDTHNS YAYDGNRVRK
181WNVTTTNYGK AWAAGDIVSC LIDLDDGTLS FCLNGVSLGT AFENLSRGLG MAYFPAISLS
241FKESVAFNFG SRPLRYPVAG YRPLQDPPSA DLVRAQRLLG CFRAVLSVEL DPVEGRLLDK
301ESSKWRLRGQ PTVLLTLAHI FHHFAPLLRK VYLVEAVLMS FLLGIVEKGT PTQAQSVVHQ
361VLDLLWLFME DYEVQDCLKQ LMMSLLRLYR FSPIVPDLGL QIHYLRLTIA ILRHEKSRKF
421LLSNVLFDVL RSVVFFYIKS PLRVEEAGLQ ELIPTTWWPH CSSREGKEST EMKEETAEER
481LRRRAYERGC QRLRKRIEVV EELQVQILKL LLDNKDDNGG EASRYIFLTK FRKFLQENAS
541GRGNMPMLCP PEYMVCFLHR LISALRYYWD EYKASNPHAS FSEEAYIPPQ VFYNGKVDYF
601DLQRLGGLLS HLRKTLKDDL ASKANIVIDP LELQSTAMDD LDEDEEPAPA MAQRPMQALA
661VGGPLPLPRP GWLSSPTLGR ANRFLSTAAV SLMTPRRPLS TSEKVKVRTL SVEQRTREDI
721EGSHWNEGLL LGRPPEEPEQ PLTENSLLEV LDGAVMMYNL SVHQQLGKMV GVSDDVNEYA
781MALRDTEDKL RRCPKRRKDI LAELTKSQKV FSEKLDHLSR RLAWVHATVY SQEKMLDIYW
841LLRVCLRTIE HGDRTGSLFA FMPEFYLSVA INSYSALKNY FGPVHSMEEL PGYEETLTRL
901AAILAKHFAD ARIVGTDIRD SLMQALASYV CYPHSLRAVE RIPEEQRIAM VRNLLAPYEQ
961RPWAQTNWIL VRLWRGCGFG YRYTRLPHLL KTKLEDANLP SLQKPCPSTL LQQHMADLLQ
1021QGPDVAPSFL NSVLNQLNWA FSEFIGMIQE IQQAAERLER NFVDSRQLKV CATCFDLSVS
1081LLRVLEMTIT LVPEIFLDWT RPTSEMLLRR LAQLLNQVLN RVTAERNLFD RVVTLRLPGL
1141ESVDHYPILV AVTGILVQLL VRGPASEREQ ATSVLLADPC FQLRSICYLL GQPEPPAPGT
1201ALPAPDRKRF SLQSYADYIS ADELAQVEQM LAHLTSASAQ AAAASLPTSE EDLCPICYAH
1261PISAVFQPCG HKSCKACINQ HLMNNKDCFF CKTTIVSVED WEKGANTSTT SSAA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1314 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.