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RNF121 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RNF121 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111841-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF121 (NM_018320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RNF121 | Ring finger protein 121
Origin Species
Human
NCBI GenBank
NM_018320
SwissProt
Q9H920

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVVEVEVG GGAAGERELD EVDMSDLSPE EQWRVEHARM HAKHRGHEAM HAEMVLILIA
61TLVVAQLLLV QWKQRHPRSY NMVTLFQMWV VPLYFTVKLH WWRFLVIWIL FSAVTAFVTF
121RATRKPLVQT TPRLVYKWFL LIYKISYATG IVGYMAVMFT LFGLNLLFKI KPEDAMDFGI
181SLLFYGLYYG VLERDFAEMC ADYMASTIGF YSESGMPTKH LSDSVCAVCG QQIFVDVSEE
241GIIENTYRLS CNHVFHEFCI RGWCIVGKKQ TCPYCKEKVD LKRMFSNPWE RPHVMYGQLL
301DWLRYLVAWQ PVIIGVVQGI NYILGLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

327 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.