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RNF10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RNF10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RNF10 antibodies →
$504.00each
Catalog Number: LS-G113644-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF10 (NM_014868), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ring finger protein 10 | RNF10 | RIE2 | KIAA0262
Origin Species
Human
NCBI GenBank
NM_014868
SwissProt
Q8N5U6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLSSPNAAA TASDMDKNSG SNSSSASSGS SKGQQPPRSA SAGPAGESKP KSDGKNSSGS
61KRYNRKRELS YPKNESFNNQ SRRSSSQKSK TFNKMPPQRG GGSSKLFSSS FNGGRRDEVA
121EAQRAEFSPA QFSGPKKINL NHLLNFTFEP RGQTGHFEGS GHGSWGKRNK WGHKPFNKEL
181FLQANCQFVV SEDQDYTAHF ADPDTLVNWD FVEQVRICSH EVPSCPICLY PPTAAKITRC
241GHIFCWACIL HYLSLSEKTW SKCPICYSSV HKKDLKSVVA TESHQYVVGD TITMQLMKRE
301KGVLVALPKS KWMNVDHPIH LGDEQHSQYS KLLLASKEQV LHRVVLEEKV ALEQQLAEEK
361HTPESCFIEA AIQELKTREE ALSGLAGSRR EVTGVVAALE QLVLMAPLAK ESVFQPRKGV
421LEYLSAFDEE TTEVCSLDTP SRPLALPLVE EEEAVSEPEP EGLPEACDDL ELADDNLKEG
481TICTESSQQE PITKSGFTRL SSSPCYYFYQ AEDGQHMFLH PVNVRCLVRE YGSLERSPEK
541ISATVVEIAG YSMSEDVRQR HRYLSHLPLT CEFSICELAL QPPVVSKETL EMFSDDIEKR
601KRQRQKKARE ERRRERRIEI EENKKQGKYP EVHIPLENLQ QFPAFNSYTC SSDSALGPTS
661TEGHGALSIS PLSRSPGSHA DFLLTPLSPT ASQGSPSFCV GSLEEDSPFP SFAQMLRVGK
721AKADVWPKTA PKKDENSLVP PAPVDSDGES DNSDRVPVPS FQNSFSQAIE AAFMKLDTPA
781TSDPLSEEKG GKKRKKQKQK LLFSTSVVHT K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

811 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.