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RINT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RINT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RINT1 antibodies →
$504.00each
Catalog Number: LS-G110582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RINT1 (NM_021930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_021930
SwissProt
Q6NUQ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MLPAGEIGAS PAAPCCSESG DERKNLEEKS DINVTVLIGS KQVSEGTDNG DLPSYVSAFI
61EKEVGNDLKS LKKLDKLIEQ RTVSKMQLEE QVLTISSEIP KRIRSALKNA EESKQFLNQF
121LEQETHLFSA INSHLLTAQP WMDDLGTMIS QIEEIERHLA YLKWISQIEE LSDNIQQYLM
181TNNVPEAAST LVSMAELDIK LQESSCTHLL GFMRATVKFW HKILKDKLTS DFEEILAQLH
241WPFIAPPQSQ TVGLSRPASA PEIYSYLETL FCQLLKLQTS DELLTEPKQL PEKYSLPASP
301SVILPIQVML TPLQKRFRYH FRGNRQTNVL SKPEWYLAQV LMWIGNHTEF LDEKIQPILD
361KVGSLVNARL EFSRGLMMLV LEKLATDIPC LLYDDNLFCH LVDEVLLFER ELHSVHGYPG
421TFASCMHILS EETCFQRWLT VERKFALQKM DSMLSSEAAW VSQYKDITDV DEMKVPDCAE
481TFMTLLLVIT DRYKNLPTAS RKLQFLELQK DLVDDFRIRL TQVMKEETRA SLGFRYCAIL
541NAVNYISTVL ADWADNVFFL QLQQAALEVF AENNTLSKLQ LGQLASMESS VFDDMINLLE
601RLKHDMLTRQ VDHVFREVKD AAKLYKKERW LSLPSQSEQA VMSLSSSACP LLLTLRDHLL
661QLEQQLCFSL FKIFWQMLVE KLDVYIYQEI ILANHFNEGG AAQLQFDMTR NLFPLFSHYC
721KRPENYFKHI KEACIVLNLN VGSALLLKDV LQSASGQLPA TAALNEVGIY KLAQQDVEIL
781LNLRTNWPNT GK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

792 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.