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RINL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RINL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RINL antibodies →
$504.00each
Catalog Number: LS-G131034-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RINL (NM_001195833), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ras and Rab interactor-like | RINL
Origin Species
Human
SwissProt
Q6ZS11

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQPEDKAPE VPTEGVRLVP PQVNKADRTP LGVLSTLEPL TRLQRTWGVW HVPELDTQDA
61EALVGLWPLG SFLVTGRDPS QALVLRSGPL PGEVNTYQIQ KIPRGVSLES SNLCMPDLPH
121LLAFLSASRD VLPRTLLLPP PTLGPRDEHT DPVQIGRVQQ DTPGKVLSIV NQLYLETHRG
181WGREQTPQET EPEAAQRHDP APRNPAPHGV SWVKGPLSPE VDHPGPALAS LLEEEEEDLE
241GKEEGREDDP EEEGPEDVLT IHVQSLVRAR SSYVARQYRS LRVRIASDSG GPHGSGDPAT
301ELLQDVRHLL TDLQDHLAKD SYIRAVFGSR GPGLPKKDED PGPALETAVC QAVLAPLKPA
361LWTRLRTLRA PELRRLRRRQ TALRAGAGPP GAQGPGPEGQ SPAPALRSRI HERLAHLHAA
421CAPRRKVALL LEVCRDVYAG LARGENQDPL GADAFLPALT EELIWSPDIG DTQLDVEFLM
481ELLDPDELRG EAGYYLTTWF GALHHIAHYQ PETDRAPRGL SSEARASLHQ WHRRRTLHRK
541DHPRAQANLP FKEPWAEETV TGTSDN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

566 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.