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RIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109667-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIN3 (NM_024832), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAB5 interacting protein 3 | RIN3 | Ras and Rab interactor 3
Origin Species
Human
NCBI GenBank
NM_024832
SwissProt
Q8TB24

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIRHAGAPAR GDPTGPVPVV GKGEEEEEED GMRLCLPANP KNCLPHRRGI SILEKLIKTC
61PVWLQLSLGQ AEVARILHRV VAGMFLVRRD SSSKQLVLCV HFPSLNESSA EVLEYTIKEE
121KSILYLEGSA LVFEDIFRLI AFYCVSRDLL PFTLRLPQAI LEASSFTDLE TIANLGLGFW
181DSSLNPPQER GKPAEPPRDR APGFPLVSSL RPTAHDANCA CEIELSVGND RLWFVNPIFI
241EDCSSALPTD QPPLGNCPAR PLPPTSDATS PTSRWAPRRP PPPPPVLPLQ PCSPAQPPVL
301PALAPAPACP LPTSPPVPAP HVTPHAPGPP DHPNQPPMMT CERLPCPTAG LGPLREEAMK
361PGAASSPLQQ VPAPPLPAKK NLPTAPPRRR VSERVSLEDQ SPGMAAEGDQ LSLPPQGTSD
421GPEDTPREST EQGQDTEVKA SDPHSMPELP RTAKQPPVPP PRKKRISRQL ASTLPAPLEN
481AELCTQAMAL ETPTPGPPRE GQSPASQAGT QHPPAQATAH SQSSPEFKGS LASLSDSLGV
541SVMATDQDSY STSSTEEELE QFSSPSVKKK PSMILGKARH RLSFASFSSM FHAFLSNNRK
601LYKKVVELAQ DKGSYFGSLV QDYKVYSLEM MARQTSSTEM LQEIRTMMTQ LKSYLLQSTE
661LKALVDPALH SEEELEAIVE SALYKCVLKP LKEAINSCLH QIHSKDGSLQ QLKENQLVIL
721ATTTTDLGVT TSVPEVPMME KILQKFTSMH KAYSPEKKIS ILLKTCKLIY DSMALGNPGK
781PYGADDFLPV LMYVLARSNL TEMLLNVEYM MELMDPALQL GEGSYYLTTT YGALEHIKSY
841DKITVTRQLS VEVQDSIHRW ERRRTLNKAR ASRSSVQDFI CVSYLEPEQQ ARTLASRADT
901QAQALCAQCA EKFAVERPQA HRLFVLVDGR CFQLADDALP HCIKGYLLRS EPKRDFHFVY
961RPLDGGGGGG GGSPPCLVVR EPNFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

985 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.