Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RIMS2 / RIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113797-100
Catalog Number: LS-G113797
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIM2(RIMS2) (NM_014677), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OBOE | Nuclear protein | Rab-3-interacting molecule 2 | Rab-3-interacting protein 3 | Rab3-interacting molecule 2 | RIM 2 | RIM2 | RIMS2 | KIAA0751 | RAB3 interacting protein 3 | RAB3IP3
- Origin Species
- Human
- NCBI GenBank
- NM_014677
- SwissProt
- Q9UQ26
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 132.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQFETLRQVC NSVLSHFHGV FSSPPNILQN ELFGQTLNNA RKRSPSVSRD QNRRYDQREE61REEYSQYATS DTAMPRSPSD YADRRFQHEP QFYEDSDHLS YRDSNRRSHR HSKEYIVDDE121DVESRDEYER QRREEEYQSR YRSDPNLARY PVKPQPYEEQ MRIHAEVSRA RHERRHSDVS181LANADLEDSR ISMLRMDRPS RQRSISERRA AMENQRSYSM ERTREAQGPS SYAQRTTNHS241PPTPRRSPLP IDRPDLRRTD SLRKQHHLDP SSAVRKTKRE KMETMLRNDS LSSDRSESVR301PPPPKPHKSK KGGKMRQISL SSSEEELAST PEYTSCDDVE IESESVSEKG DMDYNWLDHT361SWHSSEASPM SLHPVTWQPS KDGDRLIGRI LLNKRLKDGS VPRDSGAMLG LKVVGGKMTE421SGRLCAFITK VKKGSLADTV GHLRPGDEVL EWNGRLLQGA TFEEVYNIIL ESKPEPQVEL481VVSRPIGDIP RIPDSTHAQL ESSSSSFESQ KMDRPSISVT SPMSPGMLRD VPQFLSGQLS541IKLWFDKVGH QLIVTILGAK DLPSREDGRP RNPYVKIYFL PDRSDKNKRR TKTVKKTLEP601KWNQTFIYSP VHRREFRERM LEITLWDQAR VREEESEFLG EILIELETAL LDDEPHWYKL661QTHDVSSLPL PHPSPYMPRR QLHGESPTRR LQRSKRISDS EVSDYDCDDG IGVVSDYRHD721GRDLQSSTLS VPEQVMSSNH CSPSGSPHRV DVIGRTRSWS PSVPPPQSRN VEQGLRGTRT781MTGHYNTISR MDRHRVMDDH YSPDRDSHFL TLPRSRYSQT IDHHHRDGRD CEAADRQPYH841RSRSTEQRPL LERTTTRSRS TERPDTNLMR SMPSLMTGRS APPSPALSRS HPRTGSVQTS901PSSTPVAGRR GRQLPQLPPK GTLDRKAGGK KLRSTVQRST ETGLAVEMRN WMTRQASRES961TDGSMNSYSS EGNLIFPGVR LASDSQFSDF LDGLGPAQLV GRQTLATPAM GDIQVGMMDK1021KGQLEVEIIR ARGLVVKPGS KTLPAPYVKV YLLDNGVCIA KKKTKVARKT LEPLYQQLLS1081FEESPQGKVL QIIVWGDYGR MDHKSFMGVA QILLDELELS NMVIGWFKLF PPSSLVDPTL1141APLTRRASQS SLESSTGPSY SRSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1163 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.