Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RIMS1 / RIM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113551-100
Catalog Number: LS-G113551
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIM1(RIMS1) (NM_014989), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CORD7 | RAB3-interacting protein 2 | RAB3IP2 | RAB3 interacting protein 2 | RIM | Rab-3-interacting protein 2 | RIM1 | KIAA0340 | Rab-3-interacting molecule 1 | Rab3-interacting molecule 1 | RIM 1 | RIMS1
- Origin Species
- Human
- NCBI GenBank
- AB002338
- SwissProt
- Q86UR5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 188.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSAVGPRGP RPPTVPPPMQ ELPDLSHLTE EERNIIMAVM DRQKEEEEKE EAMLKCVVRD61MAKPAACKTP RNAENQPHQP SPRLHQQFES YKEQVRKIGE EARRYQGEHK DDAPTCGICH121KTKFADGCGH LCSYCRTKFC ARCGGRVSLR SNNEDKVVMW VCNLCRKQQE ILTKSGAWFF181GSGPQQTSQD GTLSDTATGA GSEVPREKKA RLQERSRSQT PLSTAAASSQ DAAPPSAPPD241RSKGAEPSQQ ALGPEQKQAS SRSRSEPPRE RKKTPGLSEQ NGKGALKSER KRVPKTSAQP301VEGAVEERER KERRESRRLE KGRSQDYPDT PEKRDEGKAA DEEKQRKEED YQTRYRSDPN361LARYPVKPPP EEQQMRMHAR VSRARHERRH SDVALPRTEA GAALPEGKAG KRAPAAARAS421PPDSPRAYSA ERTAETRAPG AKQLTNHSPP APRHGPVPAE APELKAQEPL RKQSRLDPSS481AVLMRKAKRE KVETMLRNDS LSSDQSESVR PSPPKPHRSK RGGKKRQMSV SSSEEEGVST541PEYTSCEDVE LESESVSEKG DLDYYWLDPA TWHSRETSPI SSHPVTWQPS KEGDRLIGRV601ILNKRTTMPK DSGALLGLKV VGGKMTDLGR LGAFITKVKK GSLADVVGHL RAGDEVLEWN661GKPLPGATNE EVYNIILESK SEPQVEIIVS RPIGDIPRIP ESSHPPLESS SSSFESQKME721RPSISVISPT SPGALKDAPQ VLPGQLSVKL WYDKVGHQLI VNVLQATDLP ARVDGRPRNP781YVKMYFLPDR SDKSKRRTKT VKKILEPKWN QTFVYSHVHR RDFRERMLEI TVWDQPRVQE841EESEFLGEIL IELETALLDD EPHWYKLQTH DESSLPLPQP SPFMPRRHIH GESSSKKLQR901SQRISDSDIS DYEVDDGIGV VPPVGYRSSA RESKSTTLTV PEQQRTTHHR SRSVSPHRGN961DQGKPRSRLP NVPLQRSLDE IHPTRRSRSP TRHHDASRSP VDHRTRDVDS QYLSEQDSEL1021LMLPRAKRGR SAECLHTTRH LVRHYKTLPP KMPLLQSSSH WNIYSSILPA HTKTKSVTRQ1081DISLHHECFN STVLRFTDEI LVSELQPFLD RARSASTNCL RPDTSLHSPE RERGRWSPSL1141DRRRPPSPRI QIQHASPEND RHSRKSERSS IQKQTRKGTA SDAERVLPTC LSRRGHAAPR1201ATDQPVIRGK HPARSRSSEH SSIRTLCSMH HLVPGGSAPP SPLLTRMHRQ RSPTQSPPAD1261TSFSSRRGRQ LPQVPVRSGS IEQASLVVEE RTRQMKMKVH RFKQTTGSGS SQELDREQYS1321KYNIHKDQYR SCDNVSAKSS DSDVSDVSAI SRTSSASRLS STSFMSEQSE RPRGRISSFT1381PKMQGRRMGT SGRSIMKSTS VSGEMYTLEH NDGSQSDTAV GTVGAGGKKR RSSLSAKVVA1441IVSRRSRSTS QLSQTESGHK KLKSTIQRST ETGMAAEMRK MVRQPSREST DGSINSYSSE1501GNLIFPGVRL GADSQFSDFL DGLGPAQLVG RQTLATPAMG DIQIGMEDKK GQLEVEVIRA1561RSLTQKPGSK STPAPYVKVY LLENGACIAK KKTRIARKTL DPLYQQSLVF DESPQGKVLQ1621VIVWGDYGRM DHKCFMGVAQ ILLEELDLSS MVIGWYKLFP PSSLVDPTLT PLTRRASQSS1681LESSTGPPCI RSSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1692 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.