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RIMBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RIMBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113073-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIMBP3 (NM_015672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1666 | RIM-BP3.A | RIMS-binding protein 3A | RIM-BP3 | RIMBP3 | RIMBP3.1 | RIMS binding protein 3 | RIM-BP3.1 | RIMBP3A | RIMS binding protein 3.1 | RIMS-binding protein 3.1
Origin Species
Human
NCBI GenBank
NM_015672
SwissProt
Q9UFD9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
180.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKDSPSPLG ASPKKPGCSS PAAAVLENQR RELEKLRAEL EAERAGWRAE RRRFAARERQ
61LREEAERERR QLADRLRSKW EAQRSRELRQ LQEEMQRERE AEIRQLLRWK EAEQRQLQQL
121LHRERDGVVR QARELQRQLA EELVNRGHCS RPGASEVSAA QCRCRLQEVL AQLRWQTDGE
181QAARIRYLQA ALEVERQLFL KYILAHFRGH PALSGSPDPQ AVHSLEEPLP QTSSGSCHAP
241KPACQLGSLD SLSAEVGVRS RSLGLVSSAC SSSPDGLLST HASSLDCFAP ACSRSLDSTR
301SLPKASKSEE RPSSPDTSTP GSRRLSPPPS PLPPPPPPSA HRKLSNPRGG EGSESQPCEV
361LTPSPPGLGH HELIKLNWLL AKALWVLARR CYTLQAENKQ LRRAGCPYQA DEKVKRLKVK
421RAELTGLARR LADRARKLQE TNLRAVSAPI PGESCAGLEL CQVFARQRAR DLSEQASAPL
481AKDKQIEELR QECHLLQARV ASGPCSDLHT GRGGPCTQWL NVRDLDRLQR ESQREVLRLQ
541RQLMLQQGNG GAWPEAGGQS ATCEEVRRQM LALERELDQR RRECQELGAQ AAPARRRGEE
601AETQLQAALL KNAWLAEENG RLQAKTDWVR KVEAENSEVR GHLGRACQER DASGLIAEQL
661LQQAARGQDR QQQLQRDPQK ALCDLHPSWK EIQALQCRPG HPPEQPWETS QMPESQVKGS
721RRPKFHARPE DYAVSQPNRD IQEKREASLE ESPVALGESA SVPQVSETVP ASQPLSKKTS
781SQSNSSSEGS MWATVPSSPT LDRDTASEVD DLEPDSVSLA LEMGGSAAPA APKLKIFMAQ
841YNYNPFEGPN DHPEGELPLT AGDYIYIFGD MDEDGFYEGE LEDGRRGLVP SNFVEQIPDS
901YIPGCLPAKS PDLGPSQLPA GQDEALEEDS LLSGKAQGVV DRGLCQMVRV GSKTEVATEI
961LDTKTEACQL GLLQSMGKQG LSRPLLGTKG VLRMAPMQLH LQNVTATSAN ITWVYSSHRH
1021PHVVYLDDRE HALTPAGVSC YTFQGLCPGT HYRARVEVRL PRDLLQVYWG TMSSTVTFDT
1081LLAGPPYPPL DVLVERHASP GVLVVSWLPV TIDSAGSSNG VQVTGYAVYA DGLKVCEVAD
1141ATAGSTLLEF SQLQVPLTWQ KVSVRTMSLC GESLDSVPAQ IPEDFFMCHR WPETPPFSYT
1201CGDPSTYRVT FPVCPQKLSL APPSAKASPH NPGSCGEPQA KFLEAFFEEP PRRQSPVSNL
1261GSEGECPSSG AGSQAQELAE AWEGCRKDLL FQKSPQNHRP PSVSDQPGEK ENCSQHMGTS
1321KSPAPGFIHL RTECGPRKEP CQEKAALERV LRQKQDAQGF TPPQLGASQQ YASDFHNVLK
1381EEQEALCLDL WGTERREERR EPEPHSRQGQ ALGVKRGCQL HEPSSALCPA PSAKVIKMPR
1441GGPQQLGTGA NTPARVFVAL SDYNPLVMSA NLKAAEEELV FQKRQLLRVW GSQDTHDFYL
1501SECNRQVGNI PGRLVAEMEV GTEQTDRRWR SPAQGNLPSV AHLEDFQGLT IPQGSSLVLQ
1561GNSKRLPLWT PKIMIAALDY DPGDGQMGGQ GKGRLALRAG DVVMVYGPMD DQGFYYGELG
1621GHRGLVPAHL LDHMSLHGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1639 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.