Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RIC1 / KIAA1432 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110984-100
Catalog Number: LS-G110984
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1432(RIC1) (NM_020829), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA207C16.1 | CIP150 | KIAA1432 | Protein RIC1 homolog
- Origin Species
- Human
- NCBI GenBank
- AA587926
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 150.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIAVSTANGY ILFFHITSTR GDKYLYEPVY PKGSPQMKGT PHFKEEQCAP ALNLEMRKIL61DLQAPIMSLQ SVLEDLLVAT SDGLLHLIHW EGMTNGRKAI NLCTVPFSVD LQSSRVGSFL121GFTDVHIRDM EYCATLDGFA VVFNDGKVGF ITPVSSRFTA EQLHGVWPQD VVDGTCVAVN181NKYRLMAFGC VSGSVQVYTI DNSTGAMLLS HKLELTAKQY PDIWNKTGAV KLMRWSPDNS241VVIVTWEYGG LSLWSVFGAQ LICTLGGDFA YRSDGTKKDP LKINSMSWGA EGYHLWVISG301FGSQNTEIES DLRSVVKQPS ILLFQFIKSV LTVNPCMSNQ EQVLLQGEDR LYLNCGEASQ361TQNPRSSSTH SEHKPSREKS PFADGGLESQ GLSTLLGHRH WHVVQISSTY LESNWPIRFS421AIDKLGQNIA VVGKFGFAHY SLLTKKWKLF GNITQEQNMI VTGGLAWWND FMVLACYNIN481DRQEELRVYL RTSNLDNAFA HVTKAQAETL LLSVFQDMVI VFRADCSICL YSIERKSDGP541NTTAGIQVLQ EVSMSRYIPH PFLVVSVTLT SVSTENGITL KMPQQARGAE SIMLNLAGQL601IMMQRDRSGP QIREKDSNPN NQRKLLPFCP PVVLAQSVEN VWTTCRANKQ KRHLLEALWL661SCGGAGMKVW LPLFPRDHRK PHSFLSQRIM LPFHINIYPL AVLFEDALVL GAVNDTLLYD721SLYTRNNARE QLEVLFPFCV VERTSQIYLH HILRQLLVRN LGEQALLLAQ SCATLPYFPH781VLELMLHEVL EEEATSREPI PDPLLPTVAK FITEFPLFLQ TVVHCARKTE YALWNYLFAA841VGNPKDLFEE CLMAQDLDTA ASYLIILQNM EVPAVSRQHA TLLFNTALEQ GKWDLCRHMI901RFLKAIGSGE SETPPSTPTA QEPSSSGGFE FFRNRSISLS QSAENVPASK FSLQKTLSMP961SGPSGKRWSK DSDCAENMYI DMMLWRHARR LLEDVRLKDL GCFAAQLGFE LISWLCKERT1021RAARVDNFVI ALKRLHKDFL WPLPIIPASS ISSPFKNGKY RTVGEQLLKS QSADPFLNLE1081MDAGISNIQR SQSWLSNIGP THHEIDTASS HGPQMQDAFL SPLSNKGDEC SIGSATDLTE1141SSSMVDGDWT MVDENFSTLS LTQSELEHIS MELASKGPHK SQVQLRYLLH IFMEAGCLDW1201CIVIGLILRE SSIINQILVI TQSSEVDGEM LQNIKTGLHA VDRWASTDCP GYKPFLNIIK1261PQLQKLSEIT EEQVQPDAFQ PITMGKTPEQ TSPRAEESRG SSSHGSIPQG EVGSSNMVSR1321KEEDTAQAEE EEPFQDGTYD CSVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1344 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.