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RHPN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RHPN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RHPN2 antibodies →
$504.00each
Catalog Number: LS-G108445-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rhophilin 2(RHPN2) (NM_033103), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
76 kDa RhoB effector protein | GTP-Rho-binding protein 2 | Rhophilin-2 | RHPN2 | p76RBE | RHOBP | Rhophilin 2
Origin Species
Human
SwissProt
Q8IUC4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTDALLPAAP QPLEKKNDGY FRKGCNPLAQ TGRSKLQNQR AALNQQILKA VRMRTGAENL
61LKVATNSKVR EQVRLELSFV NSDLQMLKEE LEGLNISVGV YQNTEEAFTI PLIPLGLKET
121KDVDFAVVLK DFILEHYSED GYLYEDEIAD LMDLRQACRT PSRDEAGVEL LMTYFIQLGF
181VESRFFPPTR QMGLLFTWYD SLTGVPVSQQ NLLLEKASVL FNTGALYTQI GTRCDRQTQA
241GLESAIDAFQ RAAGVLNYLK DTFTHTPSYD MSPAMLSVLV KMMLAQAQES VFEKISLPGI
301RNEFFMLVKV AQEAAKVGEV YQQLHAAMSQ APVKENIPYS WASLACVKAH HYAALAHYFT
361AILLIDHQVK PGTDLDHQEK CLSQLYDHMP EGLTPLATLK NDQQRRQLGK SHLRRAMAHH
421EESVREASLC KKLRSIEVLQ KVLCAAQERS RLTYAQHQEE DDLLNLIDAP SVVAKTEQEV
481DIILPQFSKL TVTDFFQKLG PLSVFSANKR WTPPRSIRFT AEEGDLGFTL RGNAPVQVHF
541LDPYCSASVA GAREGDYIVS IQLVDCKWLT LSEVMKLLKS FGEDEIEMKV VSLLDSTSSM
601HNKSATYSVG MQKTYSMICL AIDDDDKTDK TKKISKKLSF LSWGTNKNRQ KSASTLCLPS
661VGAARPQVKK KLPSPFSLLN SDSSWY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.