Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RHPN1 / RHOPHILIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108084-100
Catalog Number: LS-G108084
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RHPN1 (NM_052924), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GTP-Rho-binding protein 1 | KIAA1929 | Rhophilin 1 | RHPN | ODF5 | RHOPHILIN | Rhophilin-1 | RHPN1
- Origin Species
- Human
- SwissProt
- Q8TCX5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MILEERPDGA GAGEESPRLQ GCDSLTQIQC GQLQSRRAQI HQQIDKELQM RTGAENLYRA61TSNNRVRETV ALELSYVNSN LQLLKEELEE LSGGVDPGRH GSEAVTVPMI PLGLKETKEL121DWSTPLKELI SVHFGEDGAS YEAEIRELEA LRQAMRTPSR NESGLELLTA YYNQLCFLDA181RFLTPARSLG LFFHWYDSLT GVPAQQRALA FEKGSVLFNI GALHTQIGAR QDRSCTEGAR241RAMEAFQRAA GAFSLLRENF SHAPSPDMSA ASLCALEQLM MAQAQECVFE GLSPPASMAP301QDCLAQLRLA QEAAQVAAEY RLVHRTMAQP PVHDYVPVSW TALVHVKAEY FRSLAHYHVA361MALCDGSPAT EGELPTHEQV FLQPPTSSKP RGPVLPQELE ERRQLGKAHL KRAILGQEEA421LRLHALCRVL REVDLLRAVI SQTLQRSLAK YAELDREDDF CEAAEAPDIQ PKTHQKPEAR481MPRLSQGKGP DIFHRLGPLS VFSAKNRWRL VGPVHLTRGE GGFGLTLRGD SPVLIAAVIP541GSQAAAAGLK EGDYIVSVNG QPCRWWRHAE VVTELKAAGE AGASLQVVSL LPSSRLPSLG601DRRPVLLGPR GLLRSQREHG CKTPASTWAS PRPLLNWSRK AQQGKTGGCP QPCAPVKPAP661PSSLKHPGWPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
670 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.