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RHOBTB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RHOBTB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RHOBTB3 antibodies →
$504.00each
Catalog Number: LS-G113617-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RHOBTB3 (NM_014899), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0878 | RHOBTB3
Origin Species
Human
NCBI GenBank
NM_014899
SwissProt
O94955

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSIHIVALGN EGDTFHQDNQ PSGLIRTYLG RSPLVSGDES SLLLNAASTV ARPVFTEYQA
61SAFGNVKLVV HDCPVWDIFD SDWYTSRNLI GGADIIVIKY NVNDKFSFHE VKDNYIPVIK
121RALNSVPVII AAVGTRQNEE LPCTCPLCTS DRGSCVSTTE GIQLAKELGA TYLELHSLDD
181FYIGKYFGGV LEYFMIQALN QKTSEKMKKR KMSNSFHGIR PPQLEQPEKM PVLKAEASHY
241NSDLNNLLFC CQCVDVVFYN PDLKKVVEAH KIVLCAVSHV FMLLFNVKSP TDIQDSSIIR
301TTQDLFAINR DTAFPGASHE SSGNPPLRVI VKDALFCSCL SDILRFIYSG AFQWEELEED
361IRKKLKDSGD VSNVIEKVKC ILKTPGKINC LRNCKTYQAR KPLWFYNTSL KFFLNKPMLA
421DVVFEIQGTT VPAHRAILVA RCEVMAAMFN GNYMEAKSVL IPVYGVSKET FLSFLEYLYT
481DSCCPAGIFQ AMCLLICAEM YQVSRLQHIC ELFIITQLQS MPSRELASMN LDIVDLLKKA
541KFHHSDCLST WLLHFIATNY LIFSQKPEFQ DLSVEERSFV EKHRWPSNMY LKQLAEYRKY
601IHSRKCRCLV M
TRTRRLE-GFPTag-V

611 aa + 16 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.