Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RHOBTB2 / DBC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128341-100
Catalog Number: LS-G128341
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DBC2(RHOBTB2) (NM_001160037), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DBC2 | KIAA0717 | p83 | RHOBTB2
- Origin Species
- Human
- NCBI GenBank
- NM_015178
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKARSRLMDS DMDYERPNVE TIKCVVVGDN AVGKTRLICA RACNATLTQY QLLATHVPTV61WAIDQYRVCQ EVLERSRDVV DDVSVSLRLW DTFGDHHKDR RFAYGRSDVV VLCFSIANPN121SLHHVKTMWY PEIKHFCPRA PVILVGCQLD LRYADLEAVN RARRPLARPI KPNEILPPEK181GREVAKELGI PYYETSVVAQ FGIKDVFDNA IRAALISRRH LQFWKSHLRN VQRPLLQAPF241LPPKPPPPII VVPDPPSSSE ECPAHLLEDP LCADVILVLQ ERVRIFAHKI YLSTSSSKFY301DLFLMDLSEG ELGGPSEPGG THPEDHQGHS DQHHHHHHHH HGRDFLLRAA SFDVCESVDE361AGGSGPAGLR ASTSDGILRG NGTGYLPGRG RVLSSWSRAF VSIQEEMAED PLTYKSRLMV421VVKMDSSIQP GPFRAVLKYL YTGELDENER DLMHIAHIAE LLEVFDLRMM VANILNNEAF481MNQEITKAFH VRRTNRVKEC LAKGTFSDVT FILDDGTISA HKPLLISSCD WMAAMFGGPF541VESSTREVVF PYTSKSCMRA VLEYLYTGMF TSSPDLDDMK LIILANRLCL PHLVALTEQY601TVTGLMEATQ MMVDIDGDVL VFLELAQFHC AYQLADWCLH HICTNYNNVC RKFPRDMKAM661SPENQEYFEK HRWPPVWYLK EEDHYQRARK EREKEDYLHL KRQPKRRWLF WNSPSSPSSS721AASSSSPSSS SAVVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
734 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.