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RHOBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RHOBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RHOBTB1 antibodies →
$725.00each
Catalog Number: LS-G120862-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RHOBTB1 (NM_001032380), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0740 | RHOBTB1
Origin Species
Human
NCBI GenBank
NM_014836
SwissProt
O94844

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDADMDYERP NVETIKCVVV GDNAVGKTRL ICARACNTTL TQYQLLATHV PTVWAIDQYR
61VCQEVLERSR DVVDEVSVSL RLWDTFGDHH KDRRFAYGRS DVVVLCFSIA NPNSLNHVKS
121MWYPEIKHFC PRTPVILVGC QLDLRYADLE AVNRARRPLA RPIKRGDILP PEKGREVAKE
181LGLPYYETSV FDQFGIKDVF DNAIRAALIS RRHLQFWKSH LKKVQKPLLQ APFLPPKAPP
241PVIKIPECPS MGTNEAACLL DNPLCADVLF ILQDQEHIFA HRIYLATSSS KFYDLFLMEC
301EESPNGSEGA CEKEKQSRDF QGRILSVDPE EEREEGPPRI PQADQWKSSN KSLVEALGLE
361AEGAVPETQT LTGWSKGFIG MHREMQVNPI SKRMGPMTVV RMDASVQPGP FRTLLQFLYT
421GQLDEKEKDL VGLAQIAEVL EMFDLRMMVE NIMNKEAFMN QEITKAFHVR KANRIKECLS
481KGTFSDVTFK LDDGAISAHK PLLICSCEWM AAMFGGSFVE SANSEVYLPN INKISMQAVL
541DYLYTKQLSP NLDLDPLELI ALANRFCLPH LVALAEQHAV QELTKAATSG VGIDGEVLSY
601LELAQFHNAH QLAAWCLHHI CTNYNSVCSK FRKEIKSKSA DNQEYFERHR WPPVWYLKEE
661DHYQRVKRER EKEDIALNKH RSRRKWCFWN SSPAVA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

696 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.