Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RHCE / RH Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101675-100
Catalog Number: LS-G101675
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RHCE (NM_020485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Blood group Rh(CE) polypeptide | Blood group RhCcEe antigen | CD240CE | CD240CE antigen | RH | Rh polypeptide I | RHCE | Rhesus blood group CE protein | Rhesus blood group E antigen | RhIVb(J) | Rh blood group Rhce antigen | Rh blood group | CcEe antigens | Rh polypeptide 1 | Rh30a | Rh4 | Rhesus C/E antigens | Rh blood group C antigen | RhVIII | Rh blood group antigen Evans | RHC | RHE | RHIXB | RHPI | RhVI
- Origin Species
- Human
- NCBI GenBank
- NM_020485
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSKYPRSVR RCLPLCALTL EAALILLFYF FTHYDASLED QKGLVASYQV GQDLTVMAAI61GLGFLTSSFR RHSWSSVAFN LFMLALGVQW AILLDGFLSQ FPSGKVVITL FSIRLATMSA121MSVLISAGAV LGKVNLAQLV VMVLVEVTAL GTLRMVISNI FNTDYHMNLR HFYVFAAYFG181LTVAWCLPKP LPKGTEDNDQ RATIPSLSAM LGALFLWMFW PSVNSALLRS PIQRKNAMFN241TYYALAVSVV TAISGSSLAH PQRKISMTYV HSAVLAGGVA VGTSCHLIPS PWLAMVLGLV301AGLISIGGAK CLPVCCNRVL GIHHISVMHS IFSLLGLLGE ITYIVLLVLH TVWNGNGMIG361FQVLLSIGEL SLAIVIALTS GLLTGLLLNL KIWKAPHVAK YFDDQVFWKF PHLAVGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.