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RGPD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGPD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RGPD6 antibodies →
$725.00each
Catalog Number: LS-G124473-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGPD6 (NM_001123363), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001123363

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
198.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRSKADVER YVASVLGLTP SPRQKSMKGF YFAKLYYEAK EYDLAKKYIC TYINVQERDP
61KAHRFLGLLY ELEENTEKAV ECYRRSVELN PTQKDLVLKI AELLCKNDVT DGRAKYWVER
121AAKLFPGSPA IYKLKEQLLD CEGEDGWNKL FDLIQSELYV RPDDVHVNIR LVELYRSTKR
181LKDAVAHCHE AERNIALRSS LEWNSCVVQT LKEYLESLQC LESDKSDWQA TNTDLLLAYA
241NLMLLTLSTR DVQENRELLE SFDSALQSAK SSLGGNDELS ATFLEMKGHF YMYAGSLLLK
301MGQHGNNVQW RALSELAALC YLIAFQVPRP KIKLREGKAG QNLLEMMACD RLSQSGHMLL
361SLSRGKQDFL KEVVETFANK IGQSALYDAL FSSQSPKDTS FLGSDDIGKI DVQEPELEDL
421ARYDVGAIRA HNGSLQHLTW LGLQWNSLPA LPGIRKWLKQ LFHRLPHETS RLETNAPESI
481CILDLEVFLL GVVYTSHLQL KEKCNSHHSS YQPLCLPFPV CKQLCTERQK SWWDAVCTLI
541HRKAVPGNLA KLRLLVQHEI NTLRAQEKHG LQPALLVHWA KYLQKTGSGL NSFYGQLEYI
601GRSVHYWKKV LPLLKIIKKN SIPEPIDPLF KHFHSVDIQA SEIVEYEEDA HITFAMLDAV
661NGNIEDAVTA FESIKSVVSY WNLALIFHRK AEDIENDALS PEEQEECRNY LTKTRDYLIK
721IIDDGDSNLS VVKKLPVPLE SVKQMLNSVM QELEDYSEGG PLYKNGSLRN ADSEIKHSTP
781SPTKYSLSPS KSYKYSPETP PRWTEDRNSL LNMICQQVEA IKKEMQELKL NSSKSASRHR
841WPTENYGPDS VPDGYQGSQT FHGAPLTVAT TGPSVYYSQS PAYNSQYLLR PAANVTPTKG
901SSNTEFKSTK EGFSIPVSAD GFKFGISEPG NQEKKREKPL ENDTGFQAQD ISGRKKGRGV
961IFGQTSSTFT FADVAKSTSG EGFQFGKKDL NFKGFSGAGE KLFSSRYGKM ANKANTSGDF
1021EKDDDAYKTE DSDDIHFEPV VQMPEKVELV TGEEGEKVLY SQGVKLFRFD AEVRQWKERG
1081LGNLKILKNE VNGKLRMLMR REQVLKVCAN HWITTTMNLK PLSGSDRAWM WSASDFSDGD
1141AKLERLAAKF KTPELAEEFK QKFEECQRLL LDIPLQTPHK LVDTGRAAKL IQRAEEMKSG
1201LKDFKTFLTN DQTKVTEEEN KGSGTGAAGA SDTTIKPNAE NTGPTLEWDN YDLREDALDD
1261SVSSSSVHAS PLASSPVRKN LFRFDESTTG SNFSFKSALS LSKSPAKLNQ SGTSVGTDEE
1321SVVTQEEERD GQYFEPVVPL PDLVEVSSGE ENEQVVFSHR AEIYRYDKDV GQWKERGIGD
1381IKILQNYDNK QVRIVMRRDQ VLKLCANHRI TPDMSLQNMK GTERVWVWTA CDFADGERKV
1441EHLAVRFKLQ DVADSFKKIF DEAKTAQEKD SLITPHVSRS STPRESPCGK IAVAILEETT
1501RERTDVIQGD DVADAASEVE VSSTSETTTK AVVSPPKFVF VSESVKRIFS SEKSKPFVFG
1561NSSATGSLFG FSFNAPLKSN NSETSSVAQS GSESKVEPKK CELSKNSDIE QSSDSKVKNL
1621SASFPTEESS INYTFKTPEK EPPLWHAEFT KEELVQKLRS TTKSADHLNG LLREIEATNA
1681VLMEQIKLLK SEIRRLERNQ EREKSAANLE YLKNVLLQFI FLKPGSERER LLPVINTMLQ
1741LSPEEKGKLA AVAQDEEENA SRSSG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1765 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.