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RGL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120672-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGL3 (NM_001035223), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RalGDS-like 3 | RGL3
Origin Species
Human
SwissProt
Q3MIN7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERTAGKELA LAPLQDWGEE TEDGAVYSVS LRRQRSQRRS PAEGPGGSQA PSPIANTFLH
61YRTSKVRVLR AARLERLVGE LVFGDREQDP SFMPAFLATY RTFVPTACLL GFLLPPMPPP
121PPPGVEIKKT AVQDLSFNKN LRAVVSVLGS WLQDHPQDFR DPPVHSDLGS VRTFLGWAAP
181GSAEAQKAEK LLEDFLEEAE REQEEEPPQV WTGPPRVAQT SDPDSSEACA EEEEGLMPQG
241PQLLDFSVDE VAEQLTLIDL ELFSKVRLYE CLGSVWSQRD RPGAAGASPT VRATVAQFNT
301VTGCVLGSVL GAPGLAAPQR AQRLEKWIRI AQRCRELRNF SSLRAILSAL QSNPIYRLKR
361SWGAVSREPL STFRKLSQIF SDENNHLSSR EILFQEEATE GSQEEDNTPG SLPSKPPPGP
421VPYLGTFLTD LVMLDTALPD MLEGDLINFE KRRKEWEILA RIQQLQRRCQ SYTLSPHPPI
481LAALHAQNQL TEEQSYRLSR VIEPPAASCP SSPRIRRRIS LTKRLSAKLA REKSSSPSGS
541PGDPSSPTSS VSPGSPPSSP RSRDAPAGSP PASPGPQGPS TKLPLSLDLP SPRPFALPLG
601SPRIPLPAQQ SSEARVIRVS IDNDHGNLYR SILLTSQDKA PSVVRRALQK HNVPQPWACD
661YQLFQVLPGD RVLLIPDNAN VFYAMSPVAP RDFMLRRKEG TRNTLSVSPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

710 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.