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RGL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RGL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G116435-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGL2 (NM_004761), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HKE1.5 | RAB2L | RalGDS-like factor | Ras-associated protein RAB2L | RalGDS-like 2 | RGL2 | GDS-related protein | KE1.5
Origin Species
Human
NCBI GenBank
NM_004761
SwissProt
O15211

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPRPLRLLL DTSPPGGVVL SSFRSRDPEE GGGPGGLVVG GGQEEEEEEE EEAPVSVWDE
61EEDGAVFTVT SRQYRPLDPL VPMPPPRSSR RLRAGTLEAL VRHLLDTRTS GTDVSFMSAF
121LATHRAFTST PALLGLMADR LEALESHPTD ELERTTEVAI SVLSTWLASH PEDFGSEAKG
181QLDRLESFLL QTGYAAGKGV GGGSADLIRN LRSRVDPQAP DLPKPLALPG DPPADPTDVL
241VFLADHLAEQ LTLLDAELFL NLIPSQCLGG LWGHRDRPGH SHLCPSVRAT VTQFNKVAGA
301VVSSVLGATS TGEGPGEVTI RPLRPPQRAR LLEKWIRVAE ECRLLRNFSS VYAVVSALQS
361SPIHRLRAAW GEATRDSLRV FSSLCQIFSE EDNYSQSREL LVQEVKLQSP LEPHSKKAPR
421SGSRGGGVVP YLGTFLKDLV MLDAASKDEL ENGYINFDKR RKEFAVLSEL RRLQNECRGY
481NLQPDHDIQR WLQGLRPLTE AQSHRVSCEV EPPGSSDPPA PRVLRPTLVI SQWTEVLGSV
541GVPTPLVSCD RPSTGGDEAP TTPAPLLTRL AQHMKWPSVS SLDSALESSP SLHSPADPSH
601LSPPASSPRP SRGHRRSASC GSPLSGGAEE ASGGTGYGGE GSGPGASDCR IIRVQMELGE
661DGSVYKSILV TSQDKAPSVI SRVLKKNNRD SAVASEYELV QLLPGERELT IPASANVFYA
721MDGASHDFLL RQRRRSSTAT PGVTSGPSAS GTPPSEGGGG SFPRIKATGR KIARALF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

777 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.