Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RGL1 / RGL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113413-100
Catalog Number: LS-G113413
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RGL1 (NM_015149), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0959 | RalGDS-like 1 | RGL | RGL1
- Origin Species
- Human
- NCBI GenBank
- AA152068
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVKPVGEPT QEVSKFKLST KVESTGHWLV EDHVRIWEVL KTEESSIQDW GEEVEEGAVY61HVTLKRVQIQ QAANKGARWL GVEGDQLPPG HTVSQYETCK IRTIKAGTLE KLVENLLTAF121GDNDFTYISI FLSTYRGFAS TKEVLELLLD RYGNLTSPNC EEDGSQSSSE SKMVIRNAIA181SILRAWLDQC AEDFREPPHF PCLQKLLDYL TRMMPGSDPE RRAQNLLEQF QKQEVETDNG241LPNTISFSLE EEEELEGGES AEFTCFSEDL VAEQLTYMDA QLFKKVVPHH CLGCIWSRRD301KKENKHLAPT IRATISQFNT LTKCVVSTIL GGKELKTQQR AKIIEKWINI AHECRLLKNF361SSLRAIVSAL QSNSIYRLKK TWAAVPRDRM LMFEELSDIF SDHNNHLTSR ELLMKEGTSK421FANLDSSVKE NQKRTQRRLQ LQKDMGVMQG TVPYLGTFLT DLTMLDTALQ DYIEGGLINF481EKRRREFEVI AQIKLLQSAC NSYCMTPDQK FIQWFQRQQL LTEEESYALS CEIEAAADAS541TTSPKPRKSM VKRLSLLFLG SDMITSPTPT KEQPKSTASG SSGESMDSVS VSSCESNHSE601AEEGSITPMD TPDEPQKKLS ESSSSCSSIH SMDTNSSGMS SLINPLSSPP SCNNNPKIHK661RSVSVTSITS TVLPPVYNQQ NEDTCIIRIS VEDNNGNMYK SIMLTSQDKT PAVIQRAMLK721HNLDSDPAEE YELVQVISED KELVIPDSAN VFYAMNSQVN FDFILRKKNS MEEQVKLRSR781TSLTLPRTAK RGCWSNRHSK ITLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
803 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.