LSBio
RFX8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RFX8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RFX8 antibodies →
$504.00each
Catalog Number: LS-G126788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFX8 (NM_001145664), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA-binding protein RFX8 | RFX8 | Regulatory factor X 8 | Regulatory factor X | 8
Origin Species
Human
NCBI GenBank
NM_001145664
SwissProt
Q6ZV50

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MYEIYVETCG QNTENQVNPA TFGKCEDHSP MKTDPVGSPL SEFRRCPFLE QELAKKYSCN
61MMAFLADEYC NYCRDILRNV EDLLTSFWKS LQQDTVMLMS LPDVCQLFKC YDVQLYKGIE
121DVLLHDFLED VSIQYLKSVQ LFSKKFKLWL LNALEGVPAL LQISKLKEVT LFVKRLRRKT
181YLSNMAKTMR MVLKSKRRVS VLKSDLQAII NQGTLATSKK ALASDRSGAD ELENNPEMKC
241LRNLISLLGT STDLRVFLSC LSSHLQAFVF QTSRSKEEFT KLAASFQLRW NLLLTAVSKA
301MTLCHRDSFG SWHLFHLLLL EYMIHILQSC LEEEEEEEDM GTVKEMLPDD PTLGQPDQAL
361FHSLNSSLSQ ACASPSMEPL GVMPTHMGQG RYPVGVSNMV LRILGFLVDT AMGNKLIQVL
421LEDETTESAV KLSLPMGQEA LITLKDGQQF VIQISDVPQS SEDIYFRENN ANV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

473 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.