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RFX4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RFX4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RFX4 antibodies →
$504.00each
Catalog Number: LS-G108762-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFX4 (NM_032491), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Regulatory factor X 4 | Transcription factor RFX4 | NYD-SP10 | RFX4
Origin Species
Human
NCBI GenBank
NM_002920
SwissProt
Q33E94

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNWAAFGGSE FFIPEGIQID SRCPLSRNIT EWYHYYGIAV KESSQYYDVM YSKKGAAWVS
61ETGKKEVSKQ TVAYSPRSKL GTLLPEFPNV KDLNLPASLP EEKVSTFIMM YRTHCQRILD
121TVIRANFDEV QSFLLHFWQG MPPHMLPVLG SSTVVNIVGV CDSILYKAIS GVLMPTVLQA
181LPDSLTQVIR KFAKQLDEWL KVALHDLPEN LRNIKFELSR RFSQILRRQT SLNHLCQASR
241TVIHSADITF QMLEDWRNVD LNSITKQTLY TMEDSRDEHR KLITQLYQEF DHLLEEQSPI
301ESYIEWLDTM VDRCVVKVAA KRQGSLKKVA QQFLLMWSCF GTRVIRDMTL HSAPSFGSFH
361LIHLMFDDYV LYLLESLHCQ ERTNELMRAM KGEGSTAEVR EEIILTEAAA PTPSPVPSFS
421PAKSATSVEV PPPSSPVSNP SPEYTGLSTT GAMQSYTWSL TYTVTTAAGS PAENSQQLPC
481MRNTHVPSSS VTHRIPVYPH REEHGYTGSY NYGSYGNQHP HPMQSQYPAL PHDTAISGPL
541HYAPYHRSSA QYPFNSPTSR MEPCLMSSTP RLHPTPVTPR WPEVPSANTC YTSPSVHSAR
601YGNSSDMYTP LTTRRNSEYE HMQHFPGFAY INGEASTGWA K
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

641 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.