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RFTN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RFTN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106971-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFTN2 (NM_144629), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf11 | Raftlin-2 | RFTN2 | Raftlin family member 2 | Raft-linking protein 2
Origin Species
Human
SwissProt
Q52LD8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCGLRKLED PDDSSPGKIF STLKRPQVET KTEFAYEYVL LDFTLQASSN PEVIKINSIL
61DIVTKVENYY LKGYIVGAIH PVIQPVGQRK HLPASYLYRV VLLRLKLSPK NSAAPSGQRR
121PRLVIEECPL TSEAQTNDAA KELIEKINVA AKRGMKFVGF ISQHYSPSKF CNGTNHDGDI
181ESMLHVRHGS DENCRSWNEG TLSGQSSESG IEEELHHESG QYQMEQNGSP TSSKSRKGEA
241SDNKLYTVFN AFDDDSTSWA YQEGILSMKV TRKGSVISTL DADWLELTTF YYKQGLSLID
301SFVFWETSKG EHLPKSLEGF FIYEEEGSGV PGSSRKGNDA IVVEQWTVIE GCEIKTDYGP
361LLHTLAEFGW LLTSVLPTPV LRHDSEGNLA TKQIVFLQRP VMWNSAAQTP DKKASRHIKG
421EDKNKATSRS IGLDTTSSQP AESRHLPEEC RLSPSRECWT KEGRLAQHNS FSGFSSSDNV
481LRELDDGQFD QEDGVTQVTC M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

501 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.