Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RFC2 / RFC40 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117672-100
Catalog Number: LS-G117672
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFC2 (NM_002914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A1 40 kDa subunit | Activator 1 40 kDa subunit | Activator 1 subunit 2 | Replication factor C 2 | RFC2 | Rfc40 | Replication factor C subunit 2 | RF-C 40 kDa subunit
- Origin Species
- Human
- SwissProt
- P35250
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVEAVCGGA GEVEAQDSDP APAFSKAPGS AGHYELPWVE KYRPVKLNEI VGNEDTVSRL61EVFAREGNVP NIIIAGPPGT GKTTSILCLA RALLGPALKD AMLELNASND SMTDGAQQAL121RRTMEIYSKT TRFALACNAS DKIIEPIQSR CAVLRYTKLT DAQILTRLMN VIEKERVPYT181DDGLEAIIFT AQGDMRQALN NLQSTFSGFG FINSENVFKV CDEPHPLLVK EMIQHCVNAN241IDEAYKILAH LWHLGYSPED IIGNIFRVCK TFQMAEYLKL EFIKEIGYTH MKIAEGVNSL301LQMAGLLARL CQKTMAPVAS TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V
351 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.