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REPS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

REPS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all REPS2 antibodies →
$725.00each
Catalog Number: LS-G119732-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human REPS2 (NM_001080975), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
REPS2 | Partner of RalBP1 | POB1 | RalBP1-interacting protein 2
Origin Species
Human
NCBI GenBank
NM_004726
SwissProt
Q8NFH8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MEAAAAAAAA AAAAAAAGGG CGSGPPPLLL SEGEQQCYSE LFARCAGAAG GGPGSGPPEA
61ARVAPGTATA AAGPVADLFR ASQLPAETLH QITELCGAKR VGYFGPTQFY IALKLIAAAQ
121SGLPVRIESI KCELPLPRFM MSKNDGEIRF GNPAELHGTK VQIPYLTTEK NSFKRMDDED
181KQETQSPTMS PLASPPSSPP HYQRVPLSHG YSKLRSSAEQ MHPAPYEARQ PLVQPEGSSS
241GGPGTKPLRH QASLIRSFSV ERELQDNSSY PDEPWRITEE QREYYVNQFR SLQPDPSSFI
301SGSVAKNFFT KSKLSIPELS YIWELSDADC DGALTLPEFC AAFHLIVARK NGYPLPEGLP
361PTLQPEYLQA AFPKPKWDCQ LFDSYSESLP ANQQPRDLNR MEKTSVKDMA DLPVPNQDVT
421SDDKQALKST INEALPKDVS EDPATPKDSN SLKARPRSRS YSSTSIEEAM KRGEDPPTPP
481PRPQKTHSRA SSLDLNKVFQ PSVPATKSGL LPPPPALPPR PCPSQSEQVS EAELLPQLSR
541APSQAAESSP AKKDVLYSQP PSKPIRRKFR PENQATENQE PSTAASGPAS AATMKPHPTV
601QKQSSKQKKA IQTAIRKNKE ANAVLARLNS ELQQQLKEVH QERIALENQL EQLRPVTVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

659 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.