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REPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

REPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all REPS1 antibodies →
$504.00each
Catalog Number: LS-G109130-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human REPS1 (NM_031922), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RalBP1-interacting protein 1 | REPS1
Origin Species
Human
NCBI GenBank
NM_031922
SwissProt
Q96D71

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELCGATRLG YFGRSQFYIA LKLVAVAQSG FPLRVESINT VKDLPLPRFV ASKNEQESRH
61AASYSSDSEN QGSYSGVIPP PPGRGQVKKG SVSHDTVQPR TSADAQEPAS PVVSPQQSPP
121TSPHTWRKHS RHPSGGNSER PLAGPGPFWS PFGEAQSGSS AGDAVWSGHS PPPPQENWVS
181FADTPPTSTL LTMHPASVQD QTTVRTVASA TTAIEIRRQS SSYDDPWKIT DEQRQYYVNQ
241FKTIQPDLNG FIPGSAAKEF FTKSKLPILE LSHIWELSDF DKDGALTLDE FCAAFHLVVA
301RKNGYDLPEK LPESLMPKLI DLEDSADVGD QPGEVGYSGS PAEAPPSKSP SMPSLNQTWP
361ELNQSSEQWE TFSERSSSSQ TLTQFDSNIA PADPDTAIVH PVPIRMTPSK IHMQEMELKR
421TGSDHTNPTS PLLVKPSDLL EENKINSSVK FASGNTVDGY SSSDSFTSDP EQIGSNVTRQ
481RSHSGTSPDN TAPPPPPPRP QPSHSRSSSL DMNRTFTVTT GQQQAGVVAH PPAVPPRPQP
541SQAPGPAVHR PVDADGLITH TSTSPQQIPE QPNFADFSQF EVFAASNVND EQDDEAEKHP
601EVLPAEKASD PASSLRVAKT DSKTEEKTAA SAPANVSKGT TPLAPPPKPV RRRLKSEDEL
661RPEVDEHTQK TGVLAAVLAS QPSIPRSVGK DKKAIQASIR RNKETNTVLA RLNSELQQQL
721KDVLEERISL EVQLEQLRPF FPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

743 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.