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REPIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

REPIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all REPIN1 antibodies →
$504.00each
Catalog Number: LS-G113983-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AP4(REPIN1) (NM_014374), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AP4 | H_DJ0584D14.12 | REPIN1 | RIP60 | Zfp464 | Zinc finger protein AP4 | Zinc finger proten AP4 | Replication initiator 1 | ATT-binding protein | Zinc finger protein 464 | ZNF464
Origin Species
Human
NCBI GenBank
NM_013400
SwissProt
Q9BWE0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.29 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGLAQPRLLS GPSQESPQTL GKESRGLRQQ GTSVAQSGAQ APGRAHRCAH CRRHFPGWVA
61LWLHTRRCQA RLPLPCPECG RRFRHAPFLA LHRQVHAAAT PDLGFACHLC GQSFRGWVAL
121VLHLRAHSAA KRPIACPKCE RRFWRRKQLR AHLRRCHPPA PEARPFICGN CGRSFAQWDQ
181LVAHKRVHVA EALEEAAAKA LGPRPRGRPA VTAPRPGGDA VDRPFQCACC GKRFRHKPNL
241IAHRRVHTGE RPHQCPECGK RFTNKPYLTS HRRIHTGEKP YPCKECGRRF RHKPNLLSHS
301KIHKRSEGSA QAAPGPGSPQ LPAGPQESAA EPTPAVPLKP AQEPPPGAPP EHPQDPIEAP
361PSLYSCDDCG RSFRLERFLR AHQRQHTGER PFTCAECGKN FGKKTHLVAH SRVHSGERPF
421ACEECGRRFS QGSHLAAHRR DHAPDRPFVC PDCGKAFRHK PYLAAHRRIH TGEKPYVCPD
481CGKAFSQKSN LVSHRRIHTG ERPYACPDCD RSFSQKSNLI THRKSHIRDG AFCCAICGQT
541FDDEERLLAH QKKHDV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

556 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.