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RCBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RCBTB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RCBTB1 antibodies →
$725.00each
Catalog Number: LS-G111912-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLLD7(RCBTB1) (NM_018191), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLLD7 | GLP | E4.5 | RCBTB1 | RP11-185C18.1 | CLLL7
Origin Species
Human
NCBI GenBank
NM_018191
SwissProt
Q8NDN9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVDVGKWPIF TLLSPQEIAS IRKACVFGTS ASEALYVTDN DEVFVFGLNY SNCLGTGDNQ
61STLVPKKLEG LCGKKIKSLS YGSGPHVLLS TEDGVVYAWG HNGYSQLGNG TTNQGIAPVQ
121VCTNLLIKQV VEVACGSHHS MALAADGEVF AWGYNNCGQV GSGSTANQPT PRKVTNCLHI
181KRVVGIACGQ TSSMAVLDNG EVYGWGYNGN GQLGLGNNGN QLTPVRVAAL HSVCVNQIVC
241GYAHTLALTD EGLLYAWGAN TYGQLGTGNK NNLLSPAHIM VEKERVVEIA ACHSAHTSAA
301KTQGGHVYMW GQCRGQSVIL PHLTHFSCTD DVFACFATPA VSWRLLSVEH EDFLTVAESL
361KKEFDSPETA DLKFRIDGKY IHVHKAVLKI RCEHFRSMFQ SYWNEDMKEV IEIDQFSYPV
421YRAFLQYLYT DTVDLPPEDA IGLLDLATSY CENRLKKLCQ HIIKRGITVE NAFSLFSAAV
481RYDAEDLEEF CFKFCINHLT EVTQTAAFWQ MDGPLLKEFI AKASKCGAFK N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.