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RC3H2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RC3H2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RC3H2 antibodies →
$725.00each
Catalog Number: LS-G118917-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RC3H2 (NM_001100588), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MNAB | RING finger protein 164 | RC3H2 | RNF164
Origin Species
Human
NCBI GenBank
NM_018835
SwissProt
Q9HBD1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVQAAQWTE FLSCPICYNE FDENVHKPIS LGCSHTVCKT CLNKLHRKAC PFDQTAINTD
61IDVLPVNFAL LQLVGAQVPD HQSIKLSNLG ENKHYEVAKK CVEDLALYLK PLSGGKGVAS
121LNQSALSRPM QRKLVTLVNC QLVEEEGRVR AMRAARSLGE RTVTELILQH QNPQQLSANL
181WAAVRARGCQ FLGPAMQEEA LKLVLLALED GSALSRKVLV LFVVQRLEPR FPQASKTSIG
241HVVQLLYRAS CFKVTKRDED SSLMQLKEEF RSYEALRREH DAQIVHIAME AGLRISPEQW
301SSLLYGDLAH KSHMQSIIDK LQSPESFAKS VQELTIVLQR TGDPANLNRL RPHLELLANI
361DPNPDAVSPT WEQLENAMVA VKTVVHGLVD FIQNYSRKGH ETPQPQPNSK YKTSMCRDLR
421QQGGCPRGTN CTFAHSQEEL EKYRLRNKKI NATVRTFPLL NKVGVNNTVT TTAGNVISVI
481GSTETTGKIV PSTNGISNAE NSVSQLISRS TDSTLRALET VKKVGKVGAN GQNAAGPSAD
541SVTENKIGSP PKTPVSNVAA TSAGPSNVGT ELNSVPQKSS PFLTRVPVYP PHSENIQYFQ
601DPRTQIPFEV PQYPQTGYYP PPPTVPAGVA PCVPRFVRSN NVPESSLPPA SMPYADHYST
661FSPRDRMNSS PYQPPPPQPY GPVPPVPSGM YAPVYDSRRI WRPPMYQRDD IIRSNSLPPM
721DVMHSSVYQT SLRERYNSLD GYYSVACQPP SEPRTTVPLP REPCGHLKTS CEEQIRRKPD
781QWAQYHTQKA PLVSSTLPVA TQSPTPPSPL FSVDFRADFS ESVSGTKFEE DHLSHYSPWS
841CGTIGSCINA IDSEPKDVIA NSNAVLMDLD SGDVKRRVHL FETQRRTKEE DPIIPFSDGP
901IISKWGAISR SSRTGYHTTD PVQATASQGS ATKPISVSDY VPYVNAVDSR WSSYGNEATS
961SAHYVERDRF IVTDLSGHRK HSSTGDLLSL ELQQAKSNSL LLQREANALA MQQKWNSLDE
1021GRHLTLNLLS KEIELRNGEL QSDYTEDATD TKPDRDIELE LSALDTDEPD GQSEPIEEIL
1081DIQLGISSQN DQLLNGMAVE NGHPVQQHQK EPPKQKKQSL GEDHVILEEQ KTILPVTSCF
1141SQPLPVSISN ASCLPITTSV SAGNLILKTH VMSEDKNDFL KPVANGKMVN S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1191 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.