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RBMS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBMS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RBMS3 antibodies →
$504.00each
Catalog Number: LS-G129788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBMS3 (NM_001177711), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RNA-binding protein | RBMS3
Origin Species
Human
NCBI GenBank
NM_001003793
SwissProt
Q6XE24

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKRLDQPQM YPQYTYYYPH YLQTKQSYAP APHPMAPPSP STNSSSNNSS NNSSGEQLSK
61TNLYIRGLPP GTTDQDLIKL CQPYGKIVST KAILDKNTNQ CKGYGFVDFD SPAAAQKAVA
121SLKANGVQAQ MAKQQEQDPT NLYISNLPIS MDEQELENML KPFGHVISTR ILRDANGVSR
181GVGFARMEST EKCEVVIQHF NGKYLKTPPG IPAPSEPLLC KFADGGQKKR QNQSKYTQNG
241RPWPREGEAG MALTYDPTAA IQNGFYSSPY SIATNRMIPQ TSITPFIAAS PVSTYQVQST
301SWMPHPPYVM QPTGAVITPT MDHPMSMQPA NMMGPLTQQM NHLSLGTTGT YMTAAAPMQG
361TYIPQYTPVP PTAVSIEGVV ADTSPQTVAP SSQDTSGQQQ QIAVDTSNEH APAYSYQQSK
421P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

421 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.