Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RBMS2 / SCR3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117698-100
Catalog Number: LS-G117698
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBMS2 (NM_002898), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SCR3 | RBMS2 | RNA binding protein scr3
- Origin Species
- Human
- NCBI GenBank
- NM_002898
- SwissProt
- Q15434
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLSVTSRPG ISTFGYNRNN KKPYVSLAQQ MAPPSPSNST PNSSSGSNGN DQLSKTNLYI61RGLQPGTTDQ DLVKLCQPYG KIVSTKAILD KTTNKCKGYG FVDFDSPSAA QKAVTALKAS121GVQAQMAKQQ EQDPTNLYIS NLPLSMDEQE LEGMLKPFGQ VISTRILRDT SGTSRGVGFA181RMESTEKCEA IITHFNGKYI KTPPGVPAPS DPLLCKFADG GPKKRQNQGK FVQNGRAWPR241NADMGVMALT YDPTTALQNG FYPAPYNITP NRMLAQSALS PYLSSPVSSY QRVTQTSPLQ301VPNPSWMHHH SYLMQPSGSV LTPGMDHPIS LQPASMMGPL TQQLGHLSLS STGTYMPTAA361AMQGAYISQY TPVPSSSVSV EESSGQQNQV AVDAPSEHGV YSFQFNKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
407 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.