Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RBM8A / Y14 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116183-100
Catalog Number: LS-G116183
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Y14(RBM8A) (NM_005105), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Binder of OVCA1-1 | BOV-1A | C1DELq21.1 | BOV-1B | BOV-1 | DEL1q21.1 | MDS014 | Ribonucleoprotein RBM8A | Ribonucleoprotein RBM8 | RNA-binding motif protein 8A | RNA-binding protein Y14 | TAR | Y14 | ZRNP1 | RBM8 | RBM8A | RBM8B | RNA binding motif protein 8 | RNA binding motif protein 8A | RNA-binding protein 8A | BOV-1C | ZNRP | RNA binding motif protein 8B
- Origin Species
- Human
- NCBI GenBank
- NM_005105
- SwissProt
- Q9Y5S9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 19.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD61GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE121YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
174 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.