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RBM4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109181-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM4B (NM_031492), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBM30 | RNA binding motif protein 4B | RNA-binding protein 30 | ZCCHC21B | ZCCHC15 | RBM4L | RNA-binding protein 4B | RBM4B | RNA binding motif protein 30 | RNA-binding motif protein 30 | RNA-binding motif protein 4B | ZCRB3B
Origin Species
Human
NCBI GenBank
NM_031492
SwissProt
Q9BQ04

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
40 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVKLFIGNLP REATEQEIRS LFEQYGKVLE CDIIKNYGFV HIEDKTAAED AIRNLHHYKL
61HGVNINVEAS KNKSKASTKL HVGNISPTCT NQELRAKFEE YGPVIECDIV KDYAFVHMER
121AEDAVEAIRG LDNTEFQGKR MHVQLSTSRL RTAPGMGDQS GCYRCGKEGH WSKECPVDRT
181GRVADFTEQY NEQYGAVRTP YTMGYGESMY YNDAYGALDY YKRYRVRSYE AVAAAAAASA
241YNYAEQTMSH LPQVQSTTVT SHLNSTSVDP YDRHLLPNSG AAATSAAMAA AAATTSSYYG
301RDRSPLRRAA AMLPTVGEGY GYGPESELSQ ASAATRNSLY DMARYEREQY VDRARYSAFT
361RTRPLEQKLI SEEDLAANDI LDYKDDDDKV

390 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.