Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RBM45 / DRB1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105957-100
Catalog Number: LS-G105957
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM45 (NM_152945), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DRB1 | DRBP1 | RB-1 | RNA-binding protein 45 | RBM45 | RNA-binding motif protein 45 | RNA binding motif protein 45
- Origin Species
- Human
- NCBI GenBank
- NM_152945
- SwissProt
- Q8IUH3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDEAGSSASG GGFRPGVDSL DEPPNSRIFL VISKYTPESV LRERFSPFGD IQDIWVVRDK61HTKESKGIAF VKFARSSQAC RAMEEMHGQC LGPNDTKPIK VFIAQSRSSG SHRDVEDEEL121TRIFVMIPKS YTEEDLREKF KVYGDIEYCS IIKNKVTGES KGLGYVRYLK PSQAAQAIEN181CDRSFRAILA EPKNKASESS EQDYYSNMRQ EALGHEPRVN MFPFEQQSEF SSFDKNDSRG241QEAISKRLSV VSRVPFTEEQ LFSIFDIVPG LEYCEVQRDP YSNYGHGVVQ YFNVASAIYA301KYKLHGFQYP PGNRIGVSFI DDGSNATDLL RKMATQMVAA QLASMVWNNP SQQQFMQFGG361SSGSQLPQIQ TDVVLPSCKK KAPAETPVKE RLFIVFNPHP LPLDVLEDIF CRFGNLIEVY421LVSGKNVGYA KYADRISAND AIATLHGKIL NGVRLKVMLA DSPREESNKR QRTYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
474 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.