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RBM42 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RBM42 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109990-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM42 (NM_024321), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBM42 | RNA binding motif protein 42 | RNA-binding motif protein 42 | RNA-binding protein 42
Origin Species
Human
NCBI GenBank
NM_024321
SwissProt
Q9BTD8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAGAGPAPGL PGAGGPVVPG PGAGIPGKSG EERLKEMEAE MALFEQEVLG APVPGIPTAV
61PAVPTVPTVP TVEAMQVPAA PVIRPIIATN TYQQVQQTLE ARAAAAATVV PPMVGGPPFV
121GPVGFGPGDR SHLDSPEARE AMFLRRAAVA PQRAPILRPA FVPHVLQRAD SALSSAAAGP
181RPMALRPPHQ ALVGPPLPGP PGPPMMLPPM ARAPGPPLGS MAALRPPLEE PAAPRELGLG
241LGLGLKEKEE AVVAAAAGLE EASAAVAVGA GGAPAGPAVI GPSLPLALAM PLPEPEPLPL
301PLEVVRGLLP PLRIPELLSL RPRPRPPRPE PPPGLMALEV PEPLGEDKKK GKPEKLKRCI
361RTAAGSSWED PSLLEWDADD FRIFCGDLGN EVNDDILARA FSRFPSFLKA KVIRDKRTGK
421TKGYGFVSFK DPSDYVRAMR EMNGKYVGSR PIKLRKSMWK DRNLDVVRKK QKEKKKLGLR
481TRTRPLEQKL ISEEDLAAND ILDYKDDDDK V

511 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.